Review





Similar Products

proteinase k treatment  (New England Biolabs)
99
New England Biolabs proteinase k treatment
Proteinase K Treatment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pm42120501-397-28-31?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
proteinase k treatment - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
mncl 2  (MedChemExpress)
94
MedChemExpress mncl 2
Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM <t>MnCl</t> <t>2</t> for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).
Mncl 2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pmc13109723-24-34-33?v=MedChemExpress
Average 94 stars, based on 1 article reviews
mncl 2 - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
proteinase k  (New England Biolabs)
99
New England Biolabs proteinase k
Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM <t>MnCl</t> <t>2</t> for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).
Proteinase K, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pmc13107127-130-5-7?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
proteinase k - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
recombinant albumin  (New England Biolabs)
99
New England Biolabs recombinant albumin
Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM <t>MnCl</t> <t>2</t> for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).
Recombinant Albumin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pmc13109724-100-18-20?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
recombinant albumin - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
molecular biology grade dimethyl sulfoxide dmso  (Scharlau Group)
86
Scharlau Group molecular biology grade dimethyl sulfoxide dmso
Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM <t>MnCl</t> <t>2</t> for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).
Molecular Biology Grade Dimethyl Sulfoxide Dmso, supplied by Scharlau Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pm42086921-54-0-9?v=Scharlau+Group
Average 86 stars, based on 1 article reviews
molecular biology grade dimethyl sulfoxide dmso - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
biology grade molecular water  (Fisher Scientific)
86
Fisher Scientific biology grade molecular water
Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM <t>MnCl</t> <t>2</t> for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).
Biology Grade Molecular Water, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+biology+grade/pm42069942-176-4-9?v=Fisher+Scientific
Average 86 stars, based on 1 article reviews
biology grade molecular water - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

Image Search Results


Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM MnCl 2 for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).

Journal: Nucleic Acids Research

Article Title: Mitochondrial DNA replication is regulated by endoplasmic reticulum-mitochondrial contact sites, the mitochondrial calcium uniporter, and manganese

doi: 10.1093/nar/gkag233

Figure Lengend Snippet: Gene-silencing of Superoxide dismutase 2 inhibits mtDNA replication and is rescued by manganese. ( A ) si SOD2 or non-target (NT) silenced human fibroblasts were labelled with BrdU for 8 h and immunostained with anti-BrdU (green) and anti-TOMM20 (red). Scale bar = 50 µm. ( B ) Isolated protein from fibroblasts was immunoblotted for SOD2 after fractionation by SDS–PAGE, and quantified relative to VCL ( n = 4 independent experiments). ( C ) Quantification of the cytoplasmic BrdU foci per cell in 131 control (Ctrl) cells and 131 control cells supplemented with 50 µM MnCl 2 for 16 h, P = .89. 131 Ctrl versus 119 si SOD2 cells P = .03; and 119 si SOD2 cells versus 137 si SOD2 + Mn 2+ cells P = .03 ( n = 4 independent experiments). ( D ) EdU pulse-chase in primary human fibroblasts after transfection with siNT, si RTN4 , or si SOD2 . The EdU pulse was 26 h, and EdU incorporation in cytoplasmic foci was measured after chases of 0, 4, and 24 h. The chart represents the quantification of the EdU foci per cell in 26 non-target (NT) cells, 31 si RTN4 and 28 si SOD2 cells after a EdU pulse of 26 h (no chase); 46 NT cells, 57 si RTN4 , and 52 si SOD2 cells after a 4-h chase; and 41 NT, 36 si RTN4 , and 30 si SOD2 cells after a 24-h chase ( n = 3 independent experiments).

Article Snippet: All chemicals, unless otherwise stated, were purchased from Sigma–Aldrich with respective CAS number: thapsigargin (67526-95-8), tunicamycin (11089-65-9), 5-bromo-2′-deoxyuridine (BrdU) (59-14-3), 5-ethynyl-2′-deoxyuridine (EdU) (61135-33-9), EdU Click-it ® Thermo Fisher ( C10337 ), MCU-i4 (HY-138620, MedChemExpress), MnCl 2 (13446-34-9), cyclohexamide (66-81-9), actinonin (13434-13-4), HPG Click-it ® Thermo Fisher ( C10186 ).

Techniques: Isolation, Fractionation, SDS Page, Control, Pulse Chase, Transfection